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Transfection of insect cell in suspension for efficient baculovirus generation

Roest, Susan and Kapps-Fouthier, Sandra and Klopp, Julia and Rieffel, Sebastien and Gerhartz, Bernd and Shrestha, Binesh (2016) Transfection of insect cell in suspension for efficient baculovirus generation. MethodsX, 3. pp. 371-377.

Abstract

Baculovirus (BV) mediated insect cell expression system utilizes transfection as a first step to introduce recombinant baculovirus DNA into insect cells. Many labs are still relying on the conventional liposome based transfection method in adherent culture. Here we describe a more efficient method that can replace the existing method. The beauty of this method is minimal intermediate manipulation of culture during transfection and virus generation. This significantly reduces the chances of cross contamination of viruses while handling multiple targets and constructs as well as the other microbial contamination. The method is economical and doesn’t require any special adjustment in existing labs.

Item Type: Article
Keywords: Baculovirus, transfection, suspension, virus amplification
Date Deposited: 31 May 2016 23:45
Last Modified: 31 May 2016 23:45
URI: https://oak.novartis.com/id/eprint/28230

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