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Quantitative analysis of hIgG1 in monkey serum by LC-MS/MS using mass spectrometric immunoassay

Lanshoeft, Christian and Heudi, Olivier and Cianférani, Sarah and Warren, Andrew Paul and Picard, Franck and Kretz, Olivier (2016) Quantitative analysis of hIgG1 in monkey serum by LC-MS/MS using mass spectrometric immunoassay. Bioanalysis.

Abstract

Background: A sensitive LC-MS/MS method for human immunoglobulin G1 (hIgG1) quantification in monkey serum using mass spectrometric immunoassay disposable automation research tips (MSIA-D.A.R.T.s) is reported. Results: The hIgG1 was captured with biotinylated mouse anti-hIGg1 antibody targeting the fragment crystallizable (Fc) region (50.0 µg/ml) using 1000 cycles. Elution from the streptavidin coated MSIA-D.A.R.T.s was conducted with 0.4% trifluoroacetic acid in water. The method was selective and linear from 10.0-1000 ng/ml using 100 µl of serum. The method was evaluated regarding accuracy, precision, carry-over, dilution, auto-sampler stability and applied for the determination of hIgG1 concentration in monkey serum after intravitreal administration. Conclusions: The present assay is suitable for quantitative analysis of hIgG1 based therapeutic (fusion-) proteins in monkey serum at low levels.

Item Type: Article
Keywords: mass spectrometric immunoassay; immunoglobulin G1; LC-MS/MS; therapeutic proteins
Date Deposited: 26 Apr 2016 23:45
Last Modified: 26 Apr 2016 23:45
URI: https://oak.novartis.com/id/eprint/27519

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