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A Comparison of LC-MS/MS and a Fully Integrated Autosampler/Solid Phase Extraction System for the Analysis of Protein Binding Samples

Amaral, Adam and Saran, Chitra and Amin, Jakal and Hatsis, Panagiotis (2016) A Comparison of LC-MS/MS and a Fully Integrated Autosampler/Solid Phase Extraction System for the Analysis of Protein Binding Samples. Journal of Biomolecular Screening. ISSN 1552-454X

Abstract

A new analysis approach was evaluated for plasma protein binding (PPB) of small molecules using the Agilent RapidFire™ High-Throughput System coupled with a Sciex API 4000 Mass Spectrometer (RF-MS/MS). Thirty three proprietary and 12 literature compounds were subjected to rapid equilibrium dialysis (RED) and evaluated in parallel using RF-MS/MS at 20 seconds/sample and traditional LC-MS/MS at 3.5min/sample, thus making the RF-MS/MS analysis 12 times faster than LC-MS/MS. The developed high throughput analysis method showed excellent correlation with the traditional LC-MS/MS analysis method with an r2 value of 0.95. This RF-MS/MS analysis method was developed and implemented to increase sample throughput, decrease turnaround time for PPB data and time burden on existing LC-MS/MS instruments.

Item Type: Article
Keywords: Protein Binding, RapidFire™, High-Throughput, Mass Spectrometry
Date Deposited: 27 Apr 2016 23:45
Last Modified: 27 Apr 2016 23:45
URI: https://oak.novartis.com/id/eprint/26247

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