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Purifying the masses: integrating prepurification quality control, high-throughput LC/MS purification, and compound plating to feed high-throughput screening.

Isbell, John and Zhou, Yingyao and Guintu, Christina and Rynd, Matthew and Jiang, Shumei and Petrov, Dmitri and Micklash, Kenneth and Mainquist, James and Ek, Jared and Chang, Jim and Weselak, Mark and Backes, Bradley and Brailsford, Andrew and Shave, Darcy (2005) Purifying the masses: integrating prepurification quality control, high-throughput LC/MS purification, and compound plating to feed high-throughput screening. Journal of Combinatorial Chemistry, 7 (2). pp. 210-217. ISSN 1520-4766

Abstract

In this paper we report using a parallel, four-channel HPLC/MUX/MS purification system, the Purification Factory, to purify thousands of compounds destined for high-throughput screening in a single month. The maximum sample throughput during this 20-workday month was 704 samples/day. Since this purification throughput exceeded the postpurification sample and data handling capabilities provided by commercial solutions, a custom-integrated solution was designed to address these shortcomings. In this paper we detail the key improvements in automation, solvent handling, and sample handling logistics implemented to sustain a mean throughput of 528 samples/day over a multimonth time period.

Item Type: Article
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Date Deposited: 14 Dec 2009 14:02
Last Modified: 31 Jan 2013 01:22
URI: https://oak.novartis.com/id/eprint/256

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