Purifying the masses: integrating prepurification quality control, high-throughput LC/MS purification, and compound plating to feed high-throughput screening.
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Isbell, John, Zhou, Yingyao, Guintu, Christina, Rynd, Matthew, Jiang, Shumei, Petrov, Dmitri, Micklash, Kenneth, Mainquist, James, Ek, Jared, Chang, Jim, Weselak, Mark, Backes, Bradley, Brailsford, Andrew and Shave, Darcy (2005) Purifying the masses: integrating prepurification quality control, high-throughput LC/MS purification, and compound plating to feed high-throughput screening. Journal of Combinatorial Chemistry, 7 (2). pp. 210-217. ISSN 1520-4766
Abstract
In this paper we report using a parallel, four-channel HPLC/MUX/MS purification system, the Purification Factory, to purify thousands of compounds destined for high-throughput screening in a single month. The maximum sample throughput during this 20-workday month was 704 samples/day. Since this purification throughput exceeded the postpurification sample and data handling capabilities provided by commercial solutions, a custom-integrated solution was designed to address these shortcomings. In this paper we detail the key improvements in automation, solvent handling, and sample handling logistics implemented to sustain a mean throughput of 528 samples/day over a multimonth time period.
| Item Type: | Article |
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| Date Deposited: | 14 Dec 2009 14:02 |
| Last Modified: | 31 Jan 2013 01:22 |
| URI: | https://oak.novartis.com/id/eprint/256 |