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The Effect of Chirality on Common In Vitro Experiments: An Enantiomeric Pair Analysis

Bagdanoff, Jeffrey and Xu, Yongjin and Martin, Eric and Dollinger, Gavin (2015) The Effect of Chirality on Common In Vitro Experiments: An Enantiomeric Pair Analysis. Jounal of Medicinal Chemistry.

Abstract

This analysis elucidates the impact of small molecule architecture on common in vitro ADME assays. In vitro parameters considered in this analysis included Caco-2 permeability/efflux, CYP3A4 inhibition, hERG inhibition and rat microsomal extraction ratio (ER). The statistical significance and practical meaningfulness of chirality was determined by comparison of the distribution of enantiomers with the experimental variation distribution observed from duplicate measurements. Statistical tools were applied to characterize the role of molecular architecture on the outcome of a given in vitro assay. We found that CYP3A4 inhibition, hERG inhibition, Caco-2 permeability and efflux are unlikely to be modulated by chirality. However, rat microsomal ER provides a statistically significant, and quantitatively meaningful, chance of being influenced by chirality.

Item Type: Article
Date Deposited: 26 Apr 2016 23:45
Last Modified: 26 Apr 2016 23:45
URI: https://oak.novartis.com/id/eprint/25560

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