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Supercritical fluid chromatography in pharmaceutical analysis

Francotte, Eric and DESFONTAINE, Vincent and GUILLARME, Davy and Novakova, Lucie (2015) Supercritical fluid chromatography in pharmaceutical analysis. Journal of Pharmaceutical and Biomedical Analysis, 113. pp. 56-71. ISSN 07317085

Abstract

In the last few years, there has been a resurgence of supercritical fluid chromatography (SFC), which has been stimulated by the introduction of a new generation of instruments and columns from the main providers of chromatographic instrumentation, that are strongly committed to advancing the technology. The known limitations of SFC, such as weak UV sensitivity, limited reliability and poor quantitative performance have been mostly tackled with these modern instruments. In addition, due to the obvious benefits of SFC in terms of kinetic performance and its complementarity to LC, modern packed SFC columns represents today an additional strategy in the toolbox of the analytical scientist, which may be particularly interesting in pharmaceutical analysis. In the present review, the instrumentation and experimental conditions (i.e. stationary phase chemistry and dimensions, mobile phase nature, pressure and temperature) to perform "modern SFC" are discussed. The applicability of SFC in pharmaceutical analysis, including the determination of drugs in formulations and biofluids is critically discussed.

Item Type: Article
Keywords: SFC; UHPSFC; chiral separations; impurity profiling; biofluids; matrix effects; SFC-MS
Date Deposited: 26 Apr 2016 23:45
Last Modified: 26 Apr 2016 23:45
URI: https://oak.novartis.com/id/eprint/24981

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