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A novel method for immediate post-purification purity determination of fractions collected during high-throughput purification.

Yuan, Ding and Petersen, Christopher and Rynd, Matthew and Mainquist, James and Meyer, Andrew and Isbell, John (2005) A novel method for immediate post-purification purity determination of fractions collected during high-throughput purification. Journal of chromatography. A, 1092 (2). pp. 228-234. ISSN 0021-9673

Abstract

Following purification, the fractions of purified samples typically are analyzed to determine the relative purities of each fraction. We report a novel technique for performing post-purification analysis immediately after each preparative LC/MS run. The Single Pass Compound Purification and Analysis System (SPACPASS) samples and stores a representative aliquot from the fraction while it is being collected. Demonstrated for '1:1' fraction collections, this method of fraction purity assessment streamlined sample processing by reducing post-purification sample handling. For 97% of the collected fractions, this technique provided relative purities to within +/-5% when compared with more traditional post-purification analysis.

Item Type: Article
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Additional Information: author can archive post-print (ie final draft post-refereeing); Publisher's version/PDF cannot be used
Keywords: High throughput purification and analysis; High throughput purification; Fraction analysis; Single pass purification and analysis; HPLC/MS purification; LC/MS purification; Mass-directed fraction collection; Prep LC/MS; SPACPASS; Representative sampling; Representative flow stream sampling; Purity
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Date Deposited: 14 Dec 2009 14:02
Last Modified: 31 Jan 2013 01:23
URI: https://oak.novartis.com/id/eprint/242

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