Browse views: by Year, by Function, by GLF, by Subfunction, by Conference, by Journal

Insights into substrate specificity of NlpC/P60 cell wall hydrolases containing bacterial SH3 domains

Lesley, Scott, Knuth, Mark, Grant, Joanna, Farr, Carol Lynn, Xu, Qingping, Mengin-Lecreulx, Dominique, Patin, Delphine, Chiu, Hsiu-Ju, Jaroszewski, Lukasz, Godzik, Adam, Elsliger, Marc-Andre, Deacon, Ashley and Wilson, Ian (2015) Insights into substrate specificity of NlpC/P60 cell wall hydrolases containing bacterial SH3 domains. mBio, 6 (5). ISSN 2150-7511

Abstract

Bacterial SH3 (SH3b) domains are commonly fused with papain-like Nlp/P60 cell wall hydrolase domains. To understand how the modular architecture of SH3b and NlpC/P60 affects the activity of the catalytic domain, three putative NlpC/P60 cell wall hydrolases were biochemically and structurally characterized. These enzymes all have _-D-Glu-A2pm (A2pm is diaminopimelic acid) cysteine amidase (or DL-endopeptidase) activities but with different substrate specificities. One enzyme is a cell wall lysin that cleaves peptidoglycan (PG), while the other two are cell wall recycling enzymes that only cleave stem peptides with an N-terminal L-Ala. Their crystal structures revealed a highly conserved structure consisting of two SH3b domains and a C-terminal NlpC/P60 catalytic domain, despite very low sequence identity. Interestingly, loops from the first SH3b domain dock into the ends of the active site groove of the catalytic domain, remodel the substrate binding site, and modulate substrate specificity. Two amino acid differences at the domain interface alter the substrate binding specificity in favor of stem peptides in recycling enzymes, whereas the SH3b domain may extend the peptidoglycan binding surface in the cell wall lysins. Remarkably, the cell wall lysin can be converted into a recycling enzyme with a single mutation.

Item Type: Article
Date Deposited: 25 Nov 2017 00:45
Last Modified: 25 Jan 2019 00:45
URI: https://oak.novartis.com/id/eprint/24146

Search

Email Alerts

Register with OAK to receive email alerts for saved searches.