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Application of coupled affinity-sizing chromatography for the detection of proteolyzed HSA-tagged proteins

London, Anne and Patel, Kunal and Quinn, Lisa and Lemmerer, Martin (2014) Application of coupled affinity-sizing chromatography for the detection of proteolyzed HSA-tagged proteins. Protein Expression and Purification.

Abstract

Coupled affinity liquid chromatography and size exclusion chromatography (ALC-SEC) is a technique that has been shown to successfully report product quality of proteins during cell expression and prior to the commencement of downstream processing chromatography steps. This method was applied to monitoring the degradation and subsequent partial remediation of a HSA-tagged protein which showed clipping, allowing for rapid cell line development to address this product quality dilemma. This paper outlines the novel application of this method for measuring and addressing protease-induced proteolysis.

Item Type: Article
Date Deposited: 13 Oct 2015 13:12
Last Modified: 13 Oct 2015 13:12
URI: https://oak.novartis.com/id/eprint/24061

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