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Uniform isotope labeling of proteins expressed in insect cells using a custom medium

Sitarska, Agnieszka and Skora, Lukasz and Klopp, Julia and Roest, Susan and Fernandez, Cesar and Shrestha, Binesh and Gossert, Alvar (2015) Uniform isotope labeling of proteins expressed in insect cells using a custom medium. Master Thesis.

Abstract

Production of proteins uniformly labeled with 2H, 13C and 15N isotopes is essential for advanced NMR studies.
Here we present an affordable strategy for uniform isotope labeling in the insect cell system. Compared to commercial media the cost of the newly developed media are about 10-fold lower at comparable isotope incorporation. Additionally, there is no restriction on the labeling pattern (only 15N and 13C,15N are available commercially), but 2H, 13C and 15N in every combination can be labeled. The method was evaluated by the NMR studies on expressed proteins with four different uniform labeling patterns.

Item Type: Article
Date Deposited: 14 Jun 2016 23:45
Last Modified: 14 Jun 2016 23:45
URI: https://oak.novartis.com/id/eprint/23862

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