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A new microfluidic-based sampling procedure for overcoming the hematocrit problem associated with dried blood spot analysis

Leuthold, Luc Alexis and Heudi, Olivier and Déglon, Julien and Raccuglia, Marc and Augsburger, Marc and Picard, Franck and Kretz, Olivier and Thomas, Aurélien (2015) A new microfluidic-based sampling procedure for overcoming the hematocrit problem associated with dried blood spot analysis. Clinical Chemistry, 87 (4). pp. 2068-2071. ISSN 0003-27001520-6882

Abstract

Hematocrit (Hct) is one the most critical issue associated with the bioanalytical methods used for dried blood spot (DBS) sample analysis. Since Hct determines the viscosity of blood, it affects the spreading of blood onto the filter paper. Hence accurate quantitative data can only be obtained if the size of paper filter extracted contains a fixed blood volume. In the present work, we describe for the first time a microfluidic-based sampling procedure for enabling accurate blood volume collection on commercially-available DBS cards. The system allows the collection of fixed volume of blood (e.g. 5 or 10 µL) within several seconds. Reproducibility of sampling volume was examined in vivo, on capillary blood by quantifying caffeine and paraxanthine on 5 different extracted DBS spots; and in vitro, with Mavoglurant (AFQ056) on 10 different spots, at two Hct levels. The inter-spot precision data was below 8.5%, this was equivalent to that of a manually spotted volume with a calibrated pipette. Performance of this new sampling system was further assessed in term of accuracy and precision across samples (n=3) with Hct ranging from 26% to 62% and no Hct effect was observed on the obtained quantitative results. The data of the present study indicate that our novel microfluidic-based sampling procedure is accurate, precise and that the analysis of AFQ056 is not affected by Hct values. This provides a simple and standardized procedure for DBS sampling with fixed volume of capillary blood, which will eliminate the Hct issue linked to DBS sample analysis.

Item Type: Article
Keywords: quantitative analysis; dried blood spot; hematocrit effect; microfluidic; Mavoglurant.
Date Deposited: 26 Apr 2016 23:45
Last Modified: 26 Apr 2016 23:45
URI: https://oak.novartis.com/id/eprint/23788

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