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Insect cell culture in reagent bottles

Rieffel, Sebastien and Klopp, Julia and Roest, Susan and Carnal, Simon and Marti, Sabrina and Gerhartz, Bernd and Shrestha, Binesh (2014) Insect cell culture in reagent bottles. MethodsX, 1. pp. 155-161. ISSN 2215-0161

Abstract

Growing insect cells with high air space in culture vessel is common from the early development of suspension cell culture. We believed and followed it with the hope that it allows sufficient air for optimal cell growth. However, we missed to identify how much air exactly cells need for its growth and multiplication. Here we present the innovative method that changed the way we run insect cell culture. The method is easy to adapt, cost-effective and useful for both academic and industrial research labs. We believe this method will revolutionize the way we run insect cell culture by increasing throughput in a cost-effective way.

Item Type: Article
Keywords: Insect cells, expression, reagent bottle, Sf9, Sf21
Date Deposited: 13 Oct 2015 13:12
Last Modified: 08 Jul 2016 23:45
URI: https://oak.novartis.com/id/eprint/23068

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