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DNA sequencing and CRISPR-Cas9 gene editing for target validation in mammalian cells

Smurnyy, Y and Cai, M and Wu, H and McWhinnie, E and Tallarico, JA and Yang, Y and Feng, Y (2014) DNA sequencing and CRISPR-Cas9 gene editing for target validation in mammalian cells. Nat Chem Biol .

Abstract

Identification and validation of drug-resistant mutations can provide important insights into the mechanism of action of a compound. Here we demonstrate the feasibility of such an approach in mammalian cells using next-generation sequencing of drug-resistant clones and CRISPR-Cas9-mediated gene editing on two drug-target pairs, 6-thioguanine-HPRT1 and triptolide-ERCC3. We showed that disrupting functional HPRT1 allele or introducing ERCC3 point mutations by gene editing can confer drug resistance in cells

Item Type: Article
Additional Information: NIBR author: Smurnyy, Y institute: NIBR contributor address:
Date Deposited: 13 Oct 2015 13:12
Last Modified: 13 Oct 2015 13:12
URI: https://oak.novartis.com/id/eprint/22620

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