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Insect cell culture in reagent bottles

Rieffel, Sebastien, Klopp, Julia, Roest, Susan, Carnal, Simon, Marti, Sabrina, Gerhartz, Bernd and Shrestha, Binesh (2014) Insect cell culture in reagent bottles. Insect cell culture in reagent bottles, 1. pp. 155-161. ISSN 2215-0161


Growing insect cells with high air space in culture vessel is common from the early development of suspension cell culture. We believed and followed it with the hope that it allows sufficient air for optimal cell growth. However, we missed to identify how much air exactly cells need for its growth and multiplication. Here we present the innovative method that completely changed the way we run insect cell culture. The method is easy to adapt, cost-effective and useful for both academic and industrial research labs. We believe this method will revolutionize the way we run insect cell culture by increasing throughput in a cost-effective way.

Item Type: Article
Keywords: Insect cells, expression, reagent bottle, Sf9, Sf21
Date Deposited: 26 Apr 2016 23:45
Last Modified: 26 Apr 2016 23:45


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