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The HSP70 molecular chaperone is not beneficial in a mouse model of α-synucleinopathy

Shimshek, Derya and Mueller, Matthias and Wiessner, Christoph and Schweizer, Tatjana and Van Der Putten, P. Herman (2010) The HSP70 molecular chaperone is not beneficial in a mouse model of α-synucleinopathy. Public Library of Science one, 5 (4). e10014. ISSN 1932-6203

Abstract

Background
Aggregation and misfolded α-synuclein is thought to be central in the pathogenesis of Parkinson`s disease (PD). Heat-shock proteins (HSPs) that are involved in refolding and
degradation processes could lower the aggregate load of α-synuclein and thus be beneficial in α-synucleinopathies.

Methodology/Principal Findings
We co-overexpressed human A53T point-mutated α-synuclein and human HSP70 in mice, both under the control of Thy1 regulatory sequences. Behavior read-outs showed no beneficial effect of HSP70 expression in mice. In contrast, motor coordination, grip strength and weight were even worse in the α-synucleinopathy model in the presence of
HSP70 overexpression. Biochemical analyses revealed no differences in α-synuclein oligomers/aggregates, truncations and phosphorylation levels and α-synuclein localization was unchanged in immunostainings.

Conclusion/Significance
Overexpressing HSP70 in a mouse model of α-synucleinopathy did not lower the toxic load of α-synuclein species and had no benefical effect on α-synuclein-related motor deficits.

Item Type: Article
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Date Deposited: 13 Oct 2015 13:16
Last Modified: 13 Oct 2015 13:16
URI: https://oak.novartis.com/id/eprint/2208

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