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Comparative transcriptomics of early dipteran development

Jimenez-Guri, E and Huerta-Cepas, J and Cozzuto, L and Wotton, KR and Kang, H and Himmelbauer, H and Roma, G and Gabaldon, T and Jaeger, J (2013) Comparative transcriptomics of early dipteran development. BMC Genomics.

Abstract

Background: Modern sequencing technologies have massively increased the amount of data available for comparative genomics. Whole-transcriptome shotgun sequencing (RNA-seq) provides a powerful basis for comparative studies. In particular, this approach holds great promise for emerging model species in fields such as evolutionary developmental biology (evo-devo).Results: We have sequenced early embryonic transcriptomes of two non-drosophilid dipteran species: the moth midge Clogmia albipunctata, and the scuttle fly Megaselia abdita. Our analysis includes a third, published, transcriptome for the hoverfly Episyrphus balteatus. These emerging models for comparative developmental studies close an important phylogenetic gap between Drosophila melanogaster and other insect model systems. In this paper, we provide a comparative analysis of early embryonic transcriptomes across species, and use our data for a phylogenomic re-evaluation of dipteran phylogenetic relationships.Conclusions: We show how comparative transcriptomics can be used to create useful resources for evo-devo, and to investigate phylogenetic relationships. Our results demonstrate that de novo assembly of short (Illumina) reads yields high-quality, high-coverage transcriptomic data sets. We use these data to investigate deep dipteran phylogenetic relationships. Our results, based on a concatenation of 160 orthologous genes, provide support for the traditional view of Clogmia being the sister group of Brachycera (Megaselia, Episyrphus, Drosophila), rather than that of Culicomorpha (which includes mosquitoes and blackflies). 2013 Jimenez-Guri et al.; licensee BioMed Central Ltd

Item Type: Article
Additional Information: pubid: 51 nvp_institute: NIBR contributor_address: (Jimenez-Guri, Wotton, Jaeger) EMBL/CRG Research Unit in Systems Biology, Centre de Regulacio Genomica (CRG), Universitat Pompeu Fabra (UPF), Barcelona, Spain (Huerta-Cepas, Gabaldon) Bioinformatics and Genomics Programme, Centre de Regulacio Genomica (CRG), Universitat Pompeu Fabra (UPF), Barcelona, Spain (Cozzuto, Roma) CRG Bioinformatics Core, Centre de Regulacio Genomica (CRG), Universitat Pompeu Fabra (UPF), Barcelona, Spain (Kang, Himmelbauer) CRG Genomics Unit, Centre de Regulacio Genomica (CRG), Universitat Pompeu Fabra (UPF), Barcelona, Spain (Kang) Max Planck Institute for Molecular Genetics, Berlin, Germany (Kang) ICFC Life Technologies, Bld #2, 218 Yindu Road, Shanghai 200231, China (Roma) Developmental and Molecular Pathways, Novartis Institute for Biomedical Research, Basel, Switzerland (Gabaldon, Jaeger) Centre de Regulacio Genomica (CRG), Dr. Aiguader 88, Barcelona 08003, Spain
Date Deposited: 13 Oct 2015 13:13
Last Modified: 13 Oct 2015 13:13
URI: https://oak.novartis.com/id/eprint/21899

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