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Bioluminescent assays for cytochrome P450 enzymes

Auld, DS and Veith, H and Cali, JJ (2013) Bioluminescent assays for cytochrome P450 enzymes. Methods in Molecular Biology. pp. 1-9.

Abstract

The cytochrome P450 (CYP) family contains 57 enzymes in humans. The activity of CYPs against xenobiotics is a primary consideration in drug optimization efforts. Here we describe a series of bioluminescent assays that enable the rapid pro filing of CYP activity against compound collections. The assays employ a coupledenzyme format where fire fly luciferase is used to measure CYP enzyme activity through metabolism of pro-luciferase substrates. Springer Science+Business Media New York 2013

Item Type: Article
Additional Information: pubid: 30 nvp_institute: NIBR contributor_address: (Auld) Novartis Institutes for Biomedical Research, Cambridge, MA, United States (Veith) National Center for Advancing Translational Sciences, National Institutes of Health, Bethesda, MD, United States (Cali) Promega Corp., Madison, WI, United States
Date Deposited: 13 Oct 2015 13:13
Last Modified: 13 Oct 2015 13:13
URI: https://oak.novartis.com/id/eprint/21882

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