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Basement membrane invasion assays: Native basement membrane and chemoinvasion assay

Schoumacher, M and Glentis, A and Gurchenkov, VV and Vignjevic, DM (2013) Basement membrane invasion assays: Native basement membrane and chemoinvasion assay. Adhesion Protein Protocols. pp. 133-144.

Abstract

To escape the primary tumor and infiltrate stromal compartments, invasive cancer cells must traverse the basement membrane (BM). To break this dense matrix, cells develop finger-like protrusions, called invadopodia, at their ventral surface. Invadopodia secrete proteases to degrade the BM, and then elongate which allows the cell to invade the subjacent tissue. Here, we describe two complementary invasion assays. The native BM invasion assay, based on BM isolated from rat or mouse mesentery, is a physiologically significant approach for studying the stages of BM crossing at the cellular level. The Matrigel-based chemoinvasion assay is a powerful technique for studying invadopodia's molecular composition and organization at the subcellular level. Springer Science+Business Media, LLC 2013

Item Type: Article
Additional Information: pubid: 26 nvp_institute: NIBR contributor_address: (Schoumacher, Glentis, Gurchenkov, Vignjevic) Institut Curie, UMR144 CNRS, Paris, France (Schoumacher) Novartis Institute of Biomedical Research, Cambridge, MA, United States
Date Deposited: 13 Oct 2015 13:13
Last Modified: 13 Oct 2015 13:13
URI: https://oak.novartis.com/id/eprint/21878

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