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Application of phage display to high throughput antibody generation and characterization

Schofield, Darren, Pope, Anthony, Clementel, Veronica, Buckell, Jenny, Chapple, Susan, Clarke, Kay, Conquer, Jennie, Crofts, Anna, Crowther, Sandra, Dyson, Michael, Flack, Gillian, Griffin, Gareth, Hooks, Yvette, Howat, William, Kolb-Kokocinski, Anja, Kunze, Susan, Martin, Cecile, Maslen, Gareth, Mitchell, Joanne, O'Sullivan, Maureen, Perera, Rajika, Roake, Wendy, Shadbolt, Paul, Vincent, Karen, Warford, Anthony, Wilson, Wendy, Xie, Jane, Young, Joyce and McCafferty, John (2007) Application of phage display to high throughput antibody generation and characterization. Genome Biology, 8 (11). R254. ISSN 1465-6906


We have created a high quality phage display library containing over 1010 human antibodies and describe its use in the generation of antibodies on an unprecedented scale. We have selected, screened and sequenced over 38,000 recombinant antibodies to 292 antigens, yielding over 7,200 unique clones. 4,400 antibodies were characterized by specificity testing and detailed sequence analysis and the data/clones are available online. Sensitive detection was demonstrated in a bead based flow cytometry assay. Furthermore, positive staining by immunohistochemistry on tissue microarrays was found for 37% (143/381) of antibodies. Thus, we have demonstrated the potential of and illuminated the issues associated with genome-wide monoclonal antibody generation.

Item Type: Article
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Additional Information: free final full text version available at publisher's official URL and at PubMedCentral; author can archive post-print (ie final draft post-refereeing); Publisher's version/PDF may be used
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Date Deposited: 13 Oct 2015 13:17
Last Modified: 13 Oct 2015 13:17


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