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Gel filtration chromatography as a method for removing bacterial endotoxin from antibody preparations

London, Anne and Alabi, Buola and Mackay, Kasey and Lihon, Michael and He, Yaqin (2014) Gel filtration chromatography as a method for removing bacterial endotoxin from antibody preparations. Biotechnology Progress.

Abstract

The removal of bacterial endotoxins from biological samples is critical in order to avoid the potentially fatal pyrogenic response possible when introduced to mammalian systems. Endotoxins have a variety of specific characteristics that can be exploited in order to target their isolation and subsequent removal, but one that has not been extensively characterized is their difference in size from that of monoclonal antibodies. Here we present a study which utilizes gel filtration chromatography as a method for endotoxin removal from both aggregated and non-aggregated antibody preparations, outlining a mechanistically simple method for removal of this impurity.

Item Type: Article
Date Deposited: 13 Oct 2015 13:13
Last Modified: 13 Oct 2015 13:13
URI: https://oak.novartis.com/id/eprint/21430

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