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Composition and applications of focus libraries to phenotypic assays

Auld, Douglas and Wassermann, Anne and Camargo, Miguel (2015) Composition and applications of focus libraries to phenotypic assays. Frontiers in Pharmacology, 5. ISSN 1663-9812

Abstract

The wealth of bioactivity information now available on low-molecular compounds has enabled a paradigm shift in chemical biology and early phase drug discovery efforts. Traditionally chemical libraries have been most commonly employed in screening approaches where a bioassay is used to characterize a chemical library in a random search for active samples. However, robust curating of bioassay data, establishment of ontologies enabling mining of large chemical biology datasets, and a wealth of public chemical biology information has made possible the establishment of highly annotated compound collections. Such annotated chemical libraries can now be used to build a pathway/target hypothesis and have led to a new view where chemical libraries are used to characterize a bioassay. In this article we discuss the types of compounds in these annotated libraries composed of tools, probes, and drugs. As well, we provide rationale and a few examples for how such libraries can enable phenotypic/forward chemical genomic approaches. As with any approach, there are several pitfalls that need to be consider and we also outline some strategies to avoid these.

Item Type: Article
Date Deposited: 26 Apr 2016 23:45
Last Modified: 04 Jul 2016 23:45
URI: https://oak.novartis.com/id/eprint/21388

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