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Assessing the impact of engineered nanoparticles on wound healing using a novel in-vitro bioassay

Zhou, Enhua and Watson, Christa and Pizzo, Richard and Cohen, Joel and Dang, Quynh and de Barros, Pedro Macul Ferreira and Park, Chan Young and Chen, Cheng and Brain, Joseph D. and Butler, James P. and Ruberti, Jeffrey W. and Fredberg, Jeffrey J. and Demokritou, Philip (2014) Assessing the impact of engineered nanoparticles on wound healing using a novel in-vitro bioassay. Nanomedicine, 9 (18). pp. 2803-2815. ISSN 1743-58891748-6963

Abstract

As engineered nanoparticles (ENPs) increasingly enter consumer products, humans
become increasingly exposed. The first line of defense against ENPs is the epithelium,
the integrity of which can be compromised by wounds induced by trauma, infection, or
surgery, but the implications of ENPs on wound healing are poorly understood. Here we
developed an in-vitro assay to assess the impact of ENPs on the wound healing of cells
from human cornea. We show that industrially relevant ENPs impeded wound healing
and cellular migration in a manner dependent on the composition, dose and size of the
ENPs as well as cell type. CuO and ZnO ENPs impeded both viability and wound
healing for both fibroblasts and epithelial cells. Carboxylated polystyrene ENPs
retarded wound healing of corneal fibroblasts without affecting viability. Our results
highlight the impact of ENPs on cellular wound healing and provide useful tools for
studying physiological impact of ENPs.

Item Type: Article
Keywords: nanotechnology, engineered nanoparticles, high-throughput screening, corneal wound healing, nanotoxicology, nano-EHS
Date Deposited: 26 Apr 2016 23:46
Last Modified: 26 Apr 2016 23:46
URI: https://oak.novartis.com/id/eprint/21087

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