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Liquid Microjunction Surface Sampling-HPLC-MS/MS Profiling of Acetaminophen, Terfenadine and Their Metabolites in Whole-Body Rat Thin Tissue Sections

Nithya, Paranthaman and Vilmos, Kertez and Moench, Paul and Catoire, Alexandre and Flarakos, Jimmy and Gary, VanBerkel (2014) Liquid Microjunction Surface Sampling-HPLC-MS/MS Profiling of Acetaminophen, Terfenadine and Their Metabolites in Whole-Body Rat Thin Tissue Sections. Bioanalysis, 6 (19). pp. 2599-2606. ISSN 1757-6180

Abstract

ABSTRACT
Background: The aim of this work was to evaluate the analytical performance of a fully automated droplet-based surface sampling system utilizing a commercially available autosampler coupled with high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) for determining the distribution of drugs acetaminophen (APAP) and terfenadine (TER), and their metabolites in rat thin tissue sections.
Results: The mass spectral peak areas recorded were used to create heatmaps of APAP and TER and their respective metabolites sampled from dosed rat whole-body thin tissue sections. The rank order of APAP concentration observed in tissues was stomach > small intestine > liver, while the concentrations of its glucuronide and sulphate metabolites were greatest in the liver and small intestine. TER was most concentrated in the liver and kidney while its major metabolite fexofenadine was found in the liver and small intestine.
Conclusion: The spatial distributions of both drugs and their respective metabolites observed in this work were consistent with previous studies using radiolabeled drugs. Specifically, the rank order of APAP distribution found by liquid extraction-based surface sampling was identical to that determined by radio thin layer chromatography (RTLC) in rats. Likewise, the distribution of unchanged TER was in good agreement with results obtained by quantitative whole-body autoradiography (QWBA).

Item Type: Article
Date Deposited: 26 Apr 2016 23:46
Last Modified: 26 Apr 2016 23:46
URI: https://oak.novartis.com/id/eprint/20854

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