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Measurement of enzyme kinetics and inhibitor constants using enthalpy arrays

Recht, Michael I and Torres, Frank E and De Bruyker, Dirk and Bell, Alan G and Klumpp, Martin and Bruce, Richard H (2009) Measurement of enzyme kinetics and inhibitor constants using enthalpy arrays. Analytical Biochemistry, 388 (2). pp. 204-212. ISSN 1096-0309

Abstract

Enthalpy arrays enable label-free, solution-based calorimetric detection of molecular interactions in a 96-detector array format. Compared with conventional calorimetry, enthalpy arrays achieve a significant reduction of sample volume and measurement time through the combination of the small size of the detectors and ability to perform measurements in parallel. The current capabilities of the technology for studying enzyme-catalyzed reactions are demonstrated by determining the kinetic parameters for reactions with three model enzymes. In addition, the technology has been used with two classes of enzymes to determine accurate inhibitor constants for competitive inhibitors from measurements at a single inhibitor concentration.

Item Type: Article
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Additional Information: Author can archive post-print (ie final draft post-refereeing); Publisher's version/PDF cannot be used
Keywords: Nanocalorimetry; Enzyme assay; Label-free assay
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Date Deposited: 22 Feb 2010 11:49
Last Modified: 31 Jan 2013 00:49
URI: https://oak.novartis.com/id/eprint/1858

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