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Urinary prostasin in humans: relationships among prostasin, aldosterone and epithelial sodium channel activity

Koda, Aya and Wakida, Naoki and Toriyama, Kazuhiro and Yamamoto, Kazutoshi and Iijima, Hiromi and Tomita, Kimio and Kitamura, Kenichiro (2009) Urinary prostasin in humans: relationships among prostasin, aldosterone and epithelial sodium channel activity. Hypertension Research : official journal of the Japanese Society of Hypertension, 32 (4). pp. 276-281. ISSN 1348-4214

Abstract

Prostasin, a membrane-bound serine protease, is known to increase the activity of the epithelial sodium channel (ENaC). Gene expression of prostasin was shown to be regulated by aldosterone, which increases the rate of sodium reabsorption through ENaC. To clarify the physiological relationships among prostasin, aldosterone and ENaC, we developed a specific radioimmunoassay (RIA) for human prostasin. Prostasin levels in urine were determined in 26 normotensive and 121 hypertensive subjects. Aldosterone content in urine and plasma, urinary Na/K ratio and other clinical parameters were also measured. We observed a highly significant correlation between prostasin and aldosterone concentration in urine (correlation coefficient: 0.673, P<0.0001). A significant correlation was also found between urinary prostasin and plasma aldosterone concentration (correlation coefficient: 0.229, P<0.05). In addition, urinary prostasin excretion was inversely correlated with urinary Na/K ratio (correlation coefficient: -0.425, P<0.0001). In conclusion, we developed a prostasin-specific RIA and applied it to the clinical study. Our findings suggest that urinary prostasin level is strongly correlated with urinary or plasma aldosterone level and may serve as a surrogate marker for ENaC activation in hypertensive patients. However, it is not clear, at the present time, whether endogenous aldosterone regulates prostasin expression or vice versa.

Item Type: Article
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Additional Information: Author can archive post-print (ie final draft post-refereeing); Publisher's version/PDF cannot be used
Keywords: aldosterone; epithelial sodium channel; prostasin
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Date Deposited: 22 Feb 2010 11:49
Last Modified: 22 Feb 2010 11:49
URI: https://oak.novartis.com/id/eprint/1849

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