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Hybrid FMT-CT imaging of amyloid-beta plaques in a murine Alzheimer's disease model.

Hyde, Damon, de Kleine, Ruben, Maclaurin, Sarah, Miller, Eric, Brooks, Dana H, Krucker, Thomas and Ntziachristos, Vasilis (2009) Hybrid FMT-CT imaging of amyloid-beta plaques in a murine Alzheimer's disease model. NeuroImage, 44 (4). pp. 1304-1311. ISSN 1095-9572


The need to study molecular and functional parameters of Alzheimer's disease progression in animal models has led to the development of disease-specific fluorescent markers. However, curved optical interfaces and a highly heterogeneous internal structure make quantitative fluorescence imaging of the murine brain a particularly challenging tomographic problem. We investigated the integration of X-ray computed tomography (CT) information into a state-of-the-art fluorescence molecular tomography (FMT) scheme and establish that the dual-modality approach is essential for high fidelity reconstructions of distributed fluorescence within the murine brain, as compared to conventional fluorescence tomography. We employ this method in vivo using a fluorescent oxazine dye to quantify amyloid-beta plaque burden in transgenic APP23 mice modeling Alzheimer's disease. Multi-modal imaging allows for accurate signal localization and correlation of in vivo findings to ex vivo studies. The results point to FMT-CT as an essential tool for in vivo study of neurodegenerative disease in animal models and potentially humans.

Item Type: Article
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Additional Information: Author can archive post-print (ie final draft post-refereeing); Publisher's version/PDF cannot be used
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Date Deposited: 22 Feb 2010 11:50
Last Modified: 22 Feb 2010 11:50


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