Cherry-picking in an orchard: unattended LC/MS analysis from an autosampler with >32,000 samples online.
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Ari, Nader, Westling, Lucas and Isbell, John (2006) Cherry-picking in an orchard: unattended LC/MS analysis from an autosampler with >32,000 samples online. Journal of Biomolecular Screening : the official journal of the Society for Biomolecular Screening, 11 (3). pp. 318-322. ISSN 1087-0571
Abstract
The 1,536-well microplate format has widely supplanted the 384-well microplate format for high-throughput screening and for IC(50) assays. Previously, liquid chromatography/mass spectrometry (LC/MS) analyses of such samples required manual transfers of the wells of interest from a 1,536-well plate into a 384-well plate. Because this manual transfer introduced a source of potential error, it became clear that a more appropriate solution would be to sample directly from the 1,536-well plates. Currently, commercially available 1,536-well plate auto samplers are not compatible with Waters LC/MS systems. The authors have modified their CTC PAL autosampler to support injection from up to twenty-four 1,536-well plates. This allows them to cherry-pick any sample from up to 36,864 wells on the autosampler. Because of its success at this Institute, sampling from 1,536-well plates has not only become the preferred method for LC/MS analysis from IC(50) plates but also become the standard format used for the handling of and the sampling from large combinatorial libraries.
| Item Type: | Article |
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| Additional Information: | Publisher's version/PDF cannot be used |
| Keywords: | high-throughput screening, IC50 assays, LC/MS, 1536-well microplate |
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| Date Deposited: | 14 Dec 2009 14:04 |
| Last Modified: | 31 Jan 2013 01:26 |
| URI: | https://oak.novartis.com/id/eprint/158 |