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An efficient rapid system for profiling the cellular activities of molecular libraries.

Melnick, Jonathan and Janes, Jeff and Kim, Sungjoon and Chang, Jim and Sipes, Daniel and Gunderson, Andrew and Jarnes, Laura and Matzen, Jason and Garcia, Michael and Hood, Tami and Beigi, Ronak and Xia, Gang and Harig, Richard and Asatryan, Hayk and Yan, Frank and Zhou, Yingyao and Gu, Xiang-Ju and Saadat, Alham and Zhou, Vicki and King, Frederick and Shaw, Christopher and Su, Andrew and Downs, Robert and Gray, Nathanael and Schultz, Peter and Warmuth, Markus and Caldwell, Jeremy (2006) An efficient rapid system for profiling the cellular activities of molecular libraries. Proceedings of the National Academy of Sciences of the United States of America, 103 (9). pp. 3153-3158. ISSN 0027-8424

Abstract

Rapid quantitative methods for characterizing small molecules, peptides, proteins, or RNAs in a broad array of cellular assays would allow one to discover new biological activities associated with these molecules and also provide a more comprehensive profile of drug candidates early in the drug development process. Here we describe a robotic system, termed the automated compound profiler, capable of both propagating a large number of cell lines in parallel and assaying large collections of molecules simultaneously against a matrix of cellular assays in a highly reproducible manner. To illustrate its utility, we have characterized a set of 1,400 kinase inhibitors in a panel of 35 activated tyrosine-kinase-dependent cellular assays in dose-response format in a single experiment. Analysis of the resulting multidimensional dataset revealed subclusters of both inhibitors and kinases with closely correlated activities. The approach also identified activities for the p38 inhibitor BIRB796 and the dual src/abl inhibitor BMS-354825 and exposed the expected side activities for Glivec/STI571, including cellular inhibition of c-kit and platelet-derived growth factor receptor. This methodology provides a powerful tool for unraveling the cellular biology and molecular pharmacology of both naturally occurring and synthetic chemical diversity.

Item Type: Article
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Additional Information: free full text available at publisher's official URL and on PubMedCentral; author can archive post-print (ie final draft post-refereeing
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Date Deposited: 14 Dec 2009 14:04
Last Modified: 31 Jan 2013 01:26
URI: https://oak.novartis.com/id/eprint/157

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