High-Throughput Protein Production (HTPP): a review of enabling technologies to expedite protein production.
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Koehn, James and Hunt, Ian (2009) High-Throughput Protein Production (HTPP): a review of enabling technologies to expedite protein production. Methods in Molecular Biology (Clifton, N.J.), 498. pp. 1-18. ISSN 1064-3745
Abstract
Recombinant protein production plays a crucial role in the drug discovery process, contributing to several key stages of the pathway. These include exploratory research, target validation, high-throughput screening (HTS), selectivity screens, and structural biology studies. Therefore the quick and rapid production of high-quality recombinant proteins is a critical component of the successful development of therapeutic small molecule inhibitors. This chapter will therefore attempt to provide an overview of some of the current "best-in-class" cloning, expression, and purification strategies currently available that enhance protein production capabilities and enable greater throughput. As such the chapter should also enable a reader with limited understanding of the high-throughput protein production (HTPP) process with the necessary information to set up and equip a laboratory for multiparallel protein production.
| Item Type: | Article |
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| Additional Information: | author can archive post-print (ie final draft post-refereeing); Publisher's version/PDF cannot be used |
| Keywords: | Deep-well block protein expression; Miniaturized protein purification; High throughput |
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| Date Deposited: | 14 Dec 2009 13:48 |
| Last Modified: | 31 Jan 2013 00:54 |
| URI: | https://oak.novartis.com/id/eprint/1487 |