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IL-4-mediated fine tuning of IL-12p70 production by human DC.

Guenova, Emmanuella and Volz, Thomas and Sauer, Karin and Kaesler, Susanne and Müller, Martin R and Wölbing, Florian and Chen, KoMing and Schwaerzler, Christoph and Brossart, Peter and Röcken, Martin and Biedermann, Tilo (2008) IL-4-mediated fine tuning of IL-12p70 production by human DC. European Journal of Immunology, 38 (11). pp. 3138-3149. ISSN 0014-2980

Abstract

IL-4 is expressed at high levels in allergic diseases and dominates the early phases of multiple acquired immune responses. However, the precise role of IL-4 during early inflammation and its impact on the differentiation of newly recruited DC precursors remains elusive. In order to characterize the impact of IL-4 on the differentiation of human DC, we investigated the role of IL-4 on the differentiation of monocytes into DC. Human DC were differentiated from peripheral blood precursors under either low or high concentrations of IL-4. We analyzed their cytokine profile and capacity to polarize T-cell differentiation. Concentrations of 5 (low) and 50 (high) ng/mL IL-4 induced two distinct types of DC. DC differentiated under low-dose IL-4 (5 ng/mL) produced almost no IL-12p70, and primed naïve CD4+ T cells allowing IL-4 secretion and Th2 induction. In contrast, DC generated under high concentrations of IL-4 (50 ng/mL) produced large amounts of IL-12p70, low IL-10 and primed naïve CD4+ T cells to become Th1 cells. Thus, we demonstrate that the Th2 cell cytokine IL-4 decisively determines the phenotype of ongoing immune responses by orchestrating the functional phenotype of newly immigrating DC precursors.

Item Type: Article
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Additional Information: archiving not allowed on institutional repository
Keywords: Cytokine; DC; Human; Inflammation; Skin
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Date Deposited: 14 Dec 2009 13:48
Last Modified: 31 Jan 2013 00:54
URI: https://oak.novartis.com/id/eprint/1471

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