De novo DNA methylation independent establishment of maternal imprint on X chromosome in mouse oocytes.
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Chiba, Hatsune, Hirasawa, Ryutaro, Kaneda, Masahiro, Amakawa, Yuko, Li, En, Sado, Takashi and Sasaki, Hiroyuki (2008) De novo DNA methylation independent establishment of maternal imprint on X chromosome in mouse oocytes. Genesis (New York, N.Y. : 2000), 46 (12). pp. 768-774. ISSN 1526-968X
Abstract
In female mouse embryos, the paternal X chromosome (Xp) is preferentially inactivated during preimplantation development and trophoblast differentiation. This imprinted X-chromosome inactivation (XCI) is partly due to an activating imprint on the maternal X chromosome (Xm), which is set during oocyte growth. However, the nature of this imprint is unknown. DNA methylation is one candidate, and therefore we examined whether disruptions of the two de novo DNA methyltransferases in growing oocytes affect imprinted XCI. We found that accumulation of histone H3 lysine-27 trimethylation, a hallmark of XCI, occurs normally on the Xp, and not on the Xm, in female blastocysts developed from the mutant oocytes. Furthermore, the allelic expression patterns of X-linked genes including Xist and Tsix were unchanged in preimplantation embryos and also in the trophoblast. These results show that a maternal disruption of the DNA methyltransferases has no effect on imprinted XCI and argue that de novo DNA methylation is dispensable for Xm imprinting. This underscores the difference between imprinted XCI and autosomal imprinting.
| Item Type: | Article |
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| Additional Information: | archiving not allowed on institutional repository |
| Keywords: | X-chromosome inactivation; genomic imprinting; DNA methylation; oogenesis; mouse |
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| Date Deposited: | 14 Dec 2009 13:48 |
| Last Modified: | 31 Jan 2013 00:54 |
| URI: | https://oak.novartis.com/id/eprint/1451 |