Browse views: by Year, by Function, by GLF, by Subfunction, by Conference, by Journal

Inhibition of focal adhesion kinase as a potential therapeutic strategy for imatinib-resistant gastrointestinal stromal tumor.

Sakurama, Kazufumi and Noma, Kazuhiro and Takaoka, Munenori and Tomono, Yasuko and Watanabe, Nobuyuki and Hatakeyama, Shinji and Ohmori, Osamu and Hirota, Seiichi and Motoki, Takayuki and Shirakawa, Yasuhiro and Yamatsuji, Tomoki and Haisa, Minoru and Matsuoka, Junji and Tanaka, Noriaki and Naomoto, Yoshio (2009) Inhibition of focal adhesion kinase as a potential therapeutic strategy for imatinib-resistant gastrointestinal stromal tumor. Molecular Cancer Therapeutics, 8 (1). pp. 127-134. ISSN 1535-7163

Abstract

Focal adhesion kinase (FAK) is often up-regulated in a variety of malignancies, including gastrointestinal stromal tumor (GIST), and its overexpression seems to be associated with tumor progressiveness and poor prognosis. GIST is well known to have a mutation to c-KIT; thus, a specific c-KIT inhibitor (imatinib) is recognized as the first-line chemotherapy for GIST, although a certain type of c-KIT mutation reveals a resistance to imatinib due to as yet uncertain molecular mechanisms. To assess the c-KIT mutation-related variation of cellular responses to imatinib, murine lymphocyte-derived Ba/F3 cells, which are stably transduced with different types of c-KIT mutation, were treated with either imatinib or a FAK inhibitor (TAE226), and their antitumor effects were determined in vitro and in vivo. A mutation at exon 11 (KITdel559-560) displayed a high sensitivity to imatinib, whereas that at exon 17 (KIT820Tyr) showed a significant resistance to imatinib in vitro and in vivo. KIT820Tyr cells appeared to maintain the activities of FAK and AKT under the imatinib treatment, suggesting that FAK might play a role in cell survival in imatinib-resistant cells. When FAK activity in those cells was inhibited by TAE226, cell growth was equally suppressed and the cells underwent apoptosis regardless of the c-KIT mutation types. Oral administration of TAE226 significantly diminished tumor growth in nude mice bearing KIT(820Tyr) xenografts. In summary, c-KIT mutation at exon 17 displayed a resistance to imatinib with maintained activations of FAK and subsequent survival signals. Targeting FAK could be a potential therapeutic strategy for imatinib-resistant GISTs.

Item Type: Article
Additional Information: author can archive post-print (ie final draft post-refereeing); Authors final version may be deposited on institutional website/ repository if required by institution
Keywords: FAK; GIST; TAE226; c-KIT mutation; Imatinib
Date Deposited: 14 Dec 2009 13:48
Last Modified: 31 Jan 2013 00:54
URI: https://oak.novartis.com/id/eprint/1424

Search

Email Alerts

Register with OAK to receive email alerts for saved searches.