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Pore region of TRPV3 ion channel is specifically required for heat activation.

Grandl, Joerg and Hu, Hongzhen and Bandell, Michael and Bursulaya, Badry and Schmidt, Manuela and Petrus, Matthew and Patapoutian, Ardem (2008) Pore region of TRPV3 ion channel is specifically required for heat activation. Nature Neuroscience, 11 (9). pp. 1007-1013. ISSN 1546-1726

Abstract

Ion channels can be activated (gated) by a variety of stimuli, including chemicals, voltage, mechanical force or temperature. Although molecular mechanisms of ion channel gating by chemical and voltage stimuli are understood in principal, the mechanisms of temperature activation remain unknown. The transient receptor potential channel TRPV3 is a nonselective cation channel that is activated by warm temperatures and sensory chemicals such as camphor. Here we screened approcimately 14,000 random mutant clones of mouse TRPV3 and identified five single point mutations that specifically abolish heat activation but do not perturb chemical activation or voltage modulation. Notably, all five mutations are located in the putative sixth transmembrane helix and the adjacent extracellular loop in the pore region of mouse TRPV3. Although distinct in sequence, we found that the corresponding loop of frog TRPV3 is also specifically required for heat activation. These findings demonstrate that the temperature sensitivity of TRPV3 is separable from all other known activation mechanisms and implicate a specific region in temperature sensing.

Item Type: Article
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Additional Information: author can archive post-print (ie final draft post-refereeing); Publisher's version/PDF cannot be used
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Date Deposited: 14 Dec 2009 13:49
Last Modified: 31 Jan 2013 00:56
URI: https://oak.novartis.com/id/eprint/1233

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