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Photocleavage of the Polypeptide Backbone by 2-Nitrophenylalanine.

Peters, Francis B and Brock, Ansgar and Wang, Jiangyun and Schultz, Peter (2009) Photocleavage of the Polypeptide Backbone by 2-Nitrophenylalanine. Chemistry & Biology, 16 (2). pp. 148-152. ISSN 1879-1301

Abstract

Photocleavage of the polypeptide backbone is potentially a powerful and general method to activate or deactivate functional peptides and proteins with high spatial and temporal resolution. Here we show that 2-nitrophenylalanine is able to photochemically cleave the polypeptide backbone by an unusual cinnoline-forming reaction. This unnatural amino acid was genetically encoded in E. coli, and protein containing 2-nitrophenylalanine was expressed and site-specifically photocleaved.

Item Type: Article
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Keywords: Chembio
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Date Deposited: 14 Dec 2009 13:49
Last Modified: 31 Jan 2013 00:57
URI: https://oak.novartis.com/id/eprint/1220

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