Photocleavage of the Polypeptide Backbone by 2-Nitrophenylalanine.
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Peters, Francis B, Brock, Ansgar, Wang, Jiangyun and Schultz, Peter (2009) Photocleavage of the Polypeptide Backbone by 2-Nitrophenylalanine. Chemistry & Biology, 16 (2). pp. 148-152. ISSN 1879-1301
Official URL: http://www.sciencedirect.com/science?_ob=ArticleUR...
Abstract
Photocleavage of the polypeptide backbone is potentially a powerful and general method to activate or deactivate functional peptides and proteins with high spatial and temporal resolution. Here we show that 2-nitrophenylalanine is able to photochemically cleave the polypeptide backbone by an unusual cinnoline-forming reaction. This unnatural amino acid was genetically encoded in E. coli, and protein containing 2-nitrophenylalanine was expressed and site-specifically photocleaved.
Item Type: | Article |
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Keywords: | Chembio |
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Date Deposited: | 14 Dec 2009 13:49 |
Last Modified: | 31 Jan 2013 00:57 |
URI: | https://oak.novartis.com/id/eprint/1220 |