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Characterization of the yehUT two-component regulatory system of Salmonella enterica serovar Typhi and Typhimurium

Wong, Vanessa and Pickard, Derer and Barquist, Lars and Sivaraman, Karthikeyan and Hart, Peter and Arends, Mark and Holt, Kathryn and Kane, Leanne and Mottram, Lynda and Ellison, Louise and Kay, Sally and Wileman, Thomas and Kenny, Linda and Maclennan, Calman Alexander and Kingsley, Robert and Dougan, Gordon (2013) Characterization of the yehUT two-component regulatory system of Salmonella enterica serovar Typhi and Typhimurium. PLoS ONE, 8 (12). ISSN 1932-6203

Abstract

Proteins exhibiting hyper-variable sequences within a bacterial pathogen may be associated with host adaptation. Several lineages of the monophyletic pathogen Salmonella enterica serovar Typhi (S. Typhi) have accumulated non-synonymous mutations in the putative two-component regulatory system yehUT. Consequently we evaluated the function of yehUT in S. Typhi BRD948 and S. Typhimurium ST4/74. Transcriptome analysis identified the cstA gene, encoding a carbon starvation protein as the predominantly yehUT regulated gene in both these serovars. Deletion of yehUT had no detectable effect on the ability of these mutant Salmonella to invade cultured epithelial cells (S. Typhi and S. Typhimurium) or induce colitis in a murine model (S. Typhimurium only). Growth, metabolic and antimicrobial susceptibility tests identified no obvious influences of yehUT on these phenotypes. © 2013 Wong et al.

Item Type: Article
Additional Information: This is essentially a Sanger Institute project that I have contributed to through my Birmingham affiliation. there are no IP issues.
Date Deposited: 10 Mar 2018 00:45
Last Modified: 25 Jan 2019 00:46
URI: https://oak.novartis.com/id/eprint/11169

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