Konig, Renate, Chiang, Chih-Yuan, Tu, Buu, Yan, Frank, DeJesus, Paul, Romero, Angelica, Bergauer, Tobias, Orth, Anthony, Krueger, Ute, Zhou, Yingyao and Chanda, Sumit (2007) A probability-based approach for the analysis of large-scale RNAi screens. Nature Methods, 4 (10). pp. 847-849. ISSN 1548-7091

Abstract

We describe a statistical analysis methodology designed to minimize the impact of off-target activities upon large-scale RNA interference (RNAi) screens in mammalian cells. Application of this approach enhances reconfirmation rates and facilitates the experimental validation of new gene activities through the probability-based identification of multiple distinct and active small interfering RNAs (siRNAs) targeting the same gene. We further extend this approach to establish that the optimal redundancy for efficacious RNAi collections is between 4-6 siRNAs per gene.

Item Type:	Article
Related URLs:	http://www.ncbi.nlm.nih.gov/sites/entrez...
Additional Information:	author can archive post-print (ie final draft post-refereeing); Publisher's version/PDF cannot be used
Related URLs:	http://www.ncbi.nlm.nih.gov/sites/entrez...
Date Deposited:	14 Dec 2009 14:05
Last Modified:	31 Jan 2013 01:27
URI:	https://oak.novartis.com/id/eprint/108