Ettmayer, Peter, Billich, Andreas, Baumruker, Thomas, Mechtcheriakova, Diana, Schmid, Heide and Nussbaumer, Peter (2004) Fluorescence-labeled sphingosines as substrates of sphingosine kinases 1 and 2. Bioorganic & Medicinal Chemistry Letters, 14 (6). pp. 1555-1558. ISSN 0960-894X

Abstract

Fluorescently labeled d-erythro-sphingosines have been successfully synthesized in 9 and 12 steps starting from commercially available Garner aldehyde. Determining the influence of the nature, position and linkage of the label on the in vitro phosphorylation rate by sphingosine kinases 1 and 2 resulted in the identification of a pyrene- and a NBD-labeled sphingosine which are both phosphorylated with efficiency comparable to the natural substrate.

Item Type:	Article
Related URLs:	http://www.ncbi.nlm.nih.gov/sites/entrez... doi:10.1016/j.bmcl.2003.12.099
Additional Information:	author can archive post-print (ie final draft post-refereeing); Publisher's version/PDF cannot be used
Related URLs:	http://www.ncbi.nlm.nih.gov/sites/entrez... doi:10.1016/j.bmcl.2003.12.099
Date Deposited:	14 Dec 2009 14:01
Last Modified:	31 Jan 2013 01:20
URI:	https://oak.novartis.com/id/eprint/322